Epi-fluorescent Illumination Alignment Tool / Objective Lens

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Short Description

  • An objective lens with RMS thread
  • The best tool to align the pi-fluorescence light in less than 1 min
  • Has a + sign in the center for perfect alignment
  • Large field of view to tune any light which is off center
Introduction

Mercury and xenon arc lamps are now widely utilized as illumination sources for a large number of investigations in widefield fluorescence microscopy. This tool helps you  aligning and focusing the arc lamp in a Mercury or Xenon Burner and adjusting the lamp in a fluorescence microscope.

 

Bioimager offers an optional alignment accessory to facilitate the centering of the image of the lamp arc to the rear aperture of the objective. At its upper end, this accessory either has the standard Royal Microscopical Society (RMS) thread or 25-millimeter threads to accommodate newer nosepieces, and can be screwed into the nosepiece in place of an objective. In order to use the accessory, it is first placed in the nosepiece and then rotated into the light path. The lower face of the accessory has a frosted, orange-colored glass window with an inscribed crosshair. Light passing through the microscope dichromatic mirror strikes the built-in reflector of the centering screen and is reflected onto the crosshairs. As the lamp condenser knob and the centering screws on the lamp socket are manipulated, the image can be observed and translated so that it is centered to the crosshairs. The size of the image of the arc can be made bigger or smaller by manipulating the collector lens focus position. After the arc is aligned, the centering accessory can be replaced by a regular objective.

 


Features
  • An objective lens with RMS thread
  • The best tool to align any epi-fluorescence light in less than 1 min
  • Works for both upright and inverted microscopes and any light source
  • Has a + sign in the center for perfect alignment
  • Large field of view to tune any light which is off center
Specification
  • An objective lens with RMS thread
  • The best tool to align any epi-fluorescence light in less than 1 min
  • Works for both upright and inverted microscopes and any light source
  • Has a + sign in the center for perfect alignment
  • Large field of view to tune any light which is off center
Description

Epi-fluorescent Illumination Alignment Obj Lens

 

The recommended sequence for focusing and aligning any epi-fluorescent lamp is presented below. Initially, a lamp that is freshly installed and unaligned may have a variety of orientations when the arc is focused by adjusting the collector lens. An example is illustrated in Panel A, where the arc image is positioned in the upper left and the mirror image is displaced to the lower right. After aligning the arc images and adjusting the mirror position and focus, an image similar to that shown in Panel B should appear. Merging the arc image into its mirror image will result in an overlap such as the one illustrated in Panel C. Finally, when the collector lens is defocused to illuminate the entire viewfield (Panel D), an evenly distributed and symmetrical beam should result. If this is not the case, the arc should be re-focused and the alignment procedure started fresh. Visitors are encouraged to practice using the tutorial until they can easily achieve these results.

To begin alignment of the arc lamp, focus the collector lens (see below pic) to produce a sharply defined image of the bulb. Centering knobs built onto the lamphouse exterior can then be used to translate the focused arc image directly into the center of the illumination circle produced. Some lamphouses have an internal mirror system that directs a more intense illumination into the aperture. Microscopes equipped with this type of lamphouse will produce two arc images (the actual arc and its mirror image). Use the mirror centering and lamp translation knobs to position the real arc and its mirror image (which is usually less intense) side-by-side, and then use the mirror focus knob to adjust the intensities until they are approximately equal (lamphouses lacking the mirror system will also lack the adjustment knobs, so carefully check the manufacturer's instructions on lamp adjustment). Finally, use the lamp adjustment knobs to overlay the arc and mirror images as closely as possible.

 

 


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